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I am not privy to that information and can only speak to the state of the internal war and the motivations of the leadership. However, the fact that, at least, one of the military leaders has just recently been dismissed, is noteworthy.
Not only was he dismissed, but his two legs were cut off. Whether this is a ritual battle-ceremony (which is very much a possibility) or a matter of force majeure is unclear at this point. What seems clear is that Russia is grinding its way down to victory.
The same was true of World War II, the Napoleonic Wars, and World War I.
What does this mean for the United States and Ukraine?
The Ukraine is engaged in a civil war. It will be decided in the hands of the local population, and the leadership will fall under pressure (from the Russian-controlled media, the Treasury Department, and the pressure of the US and EU). However,
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We developed a simple and rapid method for detecting the conformational change induced by an aptamer-target complex, which is based on the quenching effect of a cationic reagent and fluorescent dye in a microfluidic chip. The proposed method overcomes some drawbacks of the conventional fluorescent dye-based methods (e.g., multiple washing steps and slow data acquisition). In this study, we have developed a single-reagent (the cationic reagent) detection method for the conformational switch of an ATP aptamer, using an ATP aptamer-specific fluorophore-quencher pair. The proposed method uses a microfluidic chip with integrated wall channel structures to realize high-throughput detection. A cationic reagent-stabilized pH gradient between the wall channel and the chip surface is generated, which enables the aptamer to be stabilized. Upon introducing an ATP aptamer-target complex, the ATP aptamer’s conformation changes, thereby allowing the quencher dye to interact with the fluorophore dye. The fluorescence signal is generated by the reagents that can bind
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: Methylseleninic acid
: Symmetric dimethylarginine
: Urine-spot SMA
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
The authors would like to thank the patients and caretakers of the patients who participated in this study. We also thank Ms. Gabrielle Marchand for critical comments on this manuscript.
KT designed the study, carried out sample collection, collected and analyzed the data, and drafted the manuscript. CS participated in sample collection and data collection. JF participated in the sample collection. FT gave substantial contributions to the study conception, design and coordination, and helped to draft the manuscript. All authors read and approved the final manuscript.
This study was supported by the Natural Science Research Project of Anhui Province (1808085MH195), and Science and Technology Research and Development Project of Anhui Science and Technology Department (17030701191). The funding sources had no role in the study design, data collection, data analysis, or writing of the manuscript.
The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
All of the patients’ guardians gave written informed consent for the participation of their children and the publication of this study. This study was approved by the Ethics Committee of Anhui Provincial Hospital.