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For the biodistribution of YH753, we prepared 16 mice with breast cancer and implanted them with 1 × 10^6^ MDA-MB-231-Luc xenografts in the dorsal region. After a single administration of YH753 (2 mg/kg), six mice were sacrificed for dynamic detection of luciferase activity by an IVIS 100 imaging system after i.v. injection of luciferin. Six mice were used for biodistribution and imaging at 2 h and 12 h following intravenous injection of YH753. For biodistribution, the mice will be anesthetized by inhalational isoflurane. Subsequently, the mice will be sacrificed, and blood, liver, lung, kidney, heart, spleen, and tumor will be collected for counting by flow cytometry. All the tissues will be immediately fixed in 10% buffered formalin and embedded in paraffin. The embedded tissues will be sectioned for immunohistochemistry analysis.
For dynamic observation of the biodistribution of YH753, the collected organs will be imaged and quantified using an IVIS 100 imaging system. The mice will be anesthetized by inhalational isoflurane, and maintained under anesthesia by continuous inhalation. Subsequently, the mice will be injected with luciferin (250 mg/kg) through the tail vein at 2 h prior to killing. The mice will then be imaged using the IVIS 100 system for luciferase activity. One photon per second corresponds to 5.22 × 10^9^ photons/s/cm^2^, which
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Maria is also well known for her work as a host on Spike TV’s Nude Beach and Euro Teen Moms.
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